小畠・三重研究室

研究業績

2022

  • Eiry Kobatake, Yusuke Ikeda and Masayasu Mie
    Construction of Protein Nanoparticles for Targeted Delivery of Drugs to Cancer Cells
    Mater. Adv., (2022)
    DOI: 10.1039/D2MA00419D
  • G. Wang, Y. Mashimo, E. Kobatake, M. Mie
    Development of an enhanced immunoassay based on protein nanoparticles displaying an IgG-binding domain and luciferase
    Anal. Bioanal. Chem., 414, 2079–2088 (2022)
    DOI: 10.1007/s00216-021-03842-2

2021

  • R. Jamiruddin, A. Haq, M. U. Khondoker, T. Ali, F. Ahmed Md, S. S. Khandker, I. Jawad, R. Hossain, S. Ahmed, S. R. Rahman, M. Mustafi, T. Kaitsuka, M. Mie, K. Tomizawa, E. Kobatake, M. Haque, N. Adnan
    Antibody response to the first dose of AZD1222 vaccine in COVID-19 convalescent and uninfected individuals in Bangladesh
    Exp. Rev. Vaccines, 20(12), 1651-1660, (2021)
    DOI: 10.1080/14760584.2021.1977630
  • N. Adnan, S. S. Khandker, A. Haq, M. A. Chaity, A. Khalek, A. Q. Nazim, T. Kaitsuka, K. Tomizawa, M. Mie, E. Kobatake, S. Ahmed, N. A. A Ali, M. U. Khondoker, M. Haque, M. R. Jamiruddin
    Detection of SARS-CoV-2 by antigen ELISA test is highly swayed by viral load and sample storage condition
    Exp. Rev. Anti. Infect. Ther., 1-9, (2021)
    DOI: 10.1080/14787210.2021.1976144
  • Tada, S., Ren, XL., Mao, HL., Heo, Y., Park, SH., Isoshima, T., Zhu, LP., Zhou, XY., Ito, R., Kurata, S., Osaki, M., Kobatake, E., Ito, Y
    Versatile Mitogenic and Differentiation-Inducible Layer Formation by Underwater Adhesive Polypeptides
    Adv. Sci., 8(16): 2100961, (2021)
    DOI: 10.1002/advs.202100961
  • Sil, BK., Jahan, N., Haq, MA., Oishee, MJ., Ali, T., Khandker, SS., Kobatake, E., Mie, M., Khondoker, MU., Jamiruddin, MR., Adnan, N.
    Development and performance evaluation of a rapid in-house ELISA for retrospective serosurveillance of SARS-CoV-2
    PLOS ONE, 16(2): e0246346, (2021)
    DOI: 10.1371/journal.pone.0246346
  • Jamiruddin, MR., Haq, MA., Tomizawa, K., Kobatake, E., Mie, M., Ahmed, S., Khandker, SS., Ali, T., Jahan, N., Oishee, MJ., Khondoker, MU., Sil, BK., Haque, M., Adnan, N.
    Longitudinal Antibody Dynamics Against Structural Proteins of SARS-CoV-2 in Three COVID-19 Patients Shows Concurrent Development of IgA, IgM, and IgG
    J. Inflamm. Res., 14: 2497-2506, (2021)
    DOI: 10.2147/JIR.S313188
  • Sil, BK., Jamiruddin, MR., Haq, MA., Khondoker, MU ., Jahan, N., Khandker, SS., Ali, T., Oishee, MJ., Kaitsuka, T., Mie, M., Tomizawa, K., Kobatake, E., Haque, M., Adnan, N.
    AuNP Coupled Rapid Flow-Through Dot-Blot Immuno-Assay for Enhanced Detection of SARS-CoV-2 Specific Nucleocapsid and Receptor Binding Domain IgG
    Int. J. Nanomedicine., 16: 4739-4753, (2021)
    DOI: 10.2147/IJN.S313140

2020

  • Mie, M., Hirashima, R., Mashimo, Y., Kobatake, E.
    Construction of an Enzymatically-Conjugated DNA Aptamer-Protein Hybrid Molecule for Use as a BRET-Based Biosensor
    Applied Sciences-Basel., 10(21): 7646, (2020)
    DOI: 10.3390/app10217646
  • Heo, Y., Akimoto, J., Kobatake, E., Ito, Y
    Gelation and release behavior of visible light-curable alginate
    Polym. J., 52(3): 323-332, (2020)
    DOI: 10.1038/s41428-019-0280-6
  • Akimoto, J., Park, S.J., Obuse, S., Kawamoto, M., Tamura, M., Nandakumar, A., Kobatake, E., Ito, Y.
    Synthesis of Photoreactive Poly(ethylene oxide)s for Surface Modification
    ACS Applied. Bio. Materials., 3(9): 5941-5947, (2020)
    DOI: 10.1021/acsabm.0c00628
  • Sugihara, T., Mie, M. and Kobatake, E.
    "Direct labeling of protein nanoparticles with fluorescent compounds for immunoassay applications"
    Anal. Sci., 36(3): 385-387, (2020)
    Detailed record
  • Guo, W., Mashimo, Y., Kobatake, E. and Mie, M.
    "Construction of DNA-displaying nanoparticles by enzymatic conjugation of DNA and elastin-like polypeptides using a replication initiation protein"
    Nanotechnology, 31(25): 255102, (2020)
    Detailed record
  • Mizuguchi, Y., Mashimo, Y., Mie, M. and Kobatake, E.
    "Temperature-responsive multifunctional protein hydrogels with elastin-like polypeptides for 3-D angiogenesis"
    Biomacromolecules, 21(3): 1126-1135, (2020)
    Detailed record
  • Kim, E.H., Kawamoto, M., Dharmatti, R., Kobatake, E., Ito, Y. and Miyatake, H.
    "Preparation of biphenyl-conjugated bromotyrosine for inhibition of PD-1/PD-L1 immune checkpoint interactions"
    Int. J. Mol. Sci., 21(10): 3639, (2020)
    Detailed record
  • Sharmin, A., Adnan, N., Haque, A., Mashimo, Y., Mie, M. and Kobatake, E.
    "Construction of multifunctional fusion proteins with a laminin-derived short peptide to promote neural differentiation of mouse induced pluripotent stem cells"
    J. Biomed. Mater. Res. B Appl. Biomater., 108(6): 2691-2698, (2020)
    Detailed record
  • Park, S.J., Akimoto, J., Sakakibara, N., Kobatake, E. and Ito, Y.
    "Thermally induced switch of coupling reaction using morphological change of thermoresponsive polymer on reactive hetero-armed nanoparticle"
    ACS Appl. Mater. Interfaces, 12(43): 49165-49173, (2020)
    Detailed record
  • Kim, E.H., Ning, B., Kawamoto, M., Miyatake, H., Kobatake, E., Ito, Y. and Akimoto, J.
    "Conjugation of biphenyl groups with poly(ethylene glycol) to enhance inhibitory effects on the PD-1/PD-L1 immune checkpoint interaction"
    J. Mater. Chem. B, 8: 10162-10171, (2020)
    Detailed record
  • Seki, T., Nagaoka, M., Goto, M., Kobatake, E. and Akaike, T.
    "Direct visualization of the extracellular binding structure of E-cadherins in liquid"
    Sci. Rep., 10: 17044, (2020)
    Detailed record

2019

  • Mie, M., Matsumoto, R., Mashimo, Y., Cass, A.E.G. and Kobatake, E.
    "Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting"
    Mol. Biol. Rep., 46(1): 261-269, (2019)
    Detailed record
  • Mie, M., Niimi, T., Mashimo, Y. and Kobatake, E.
    "Construction of DNA-NanoLuc luciferase conjugates for DNA aptamer-based sandwich assay using Rep protein"
    Biotechnol. Lett., online: 1-6, (2019)
    Detailed record

2018

  • Mashimo, Y., Yoshioka, M., Tokunaga, Y., Fockenberg, C., Terada, S., Koyama, Y., Shibata-Seki, T., Yoshimoto, K., Sakai, R., Hakariya, H., Liu, L., Akaike, T., Kobatake, E., How, S.E., Uesugi, M., Chen, Y. and Kamei K.I.
    "Fabrication of a multiplexed artificial cellular microenvironment array"
    J. Vis. Exp., (139): 57377, (2018)
    Detailed record
  • Mie, M., Matsumoto, R., Mashimo, Y., Cass, A.E.G. and Kobatake, E.
    "Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting"
    Mol. Biol. Rep., online: 1-9, (2018)
    Detailed record
  • Sugihara, T., Mie, M. and Kobatake, E.
    "Application of elastin-based nanoparticles displaying antibody binding domains for a homogeneous immunoassay"
    Anal. Biochem., 544: 72-79, (2018)
    Detailed record
  • Mashimo, Y., Mie, M. and Kobatake, E.
    "A DNA-scaffold platform enhances a multi-enzymatic cycling reaction"
    Biotechnol. Lett., 40(4): 667–672, (2018)
    Detailed record

2017

  • Mizuguchi, Y., Mashimo, Y., Mie, M. and Kobatake, E.
    "Design of bFGF-tethered self-assembling extracellular matrix proteins via coiled-coil triple-helix formation"
    Biomed. Mater., 12(4): 045021, (2017)
    Detailed record
  • Usui, K., Mie, M., Andou, T., Mihara, H. and Kobatake, E.
    "Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation"
    J. Pept. Sci., 23(7-8): 659-665, (2017)
    Detailed record
  • Kamei, K., Mashimo, Y., Yoshioka, M., Tokunaga, Y., Fockenberg, C., Terada, S., Koyama, Y., Nakajima, M., Shibata‐Seki, T., Liu, L., Akaike, T., Kobatake, E., How, S. E., Uesugi, M. and Chen, Y.
    "Microfluidic‐nanofiber hybrid array for screening of cellular microenvironments"
    Small, 13(18): 1603104, (2017)
    Detailed record

2016

  • Ikeda, Y., Mashimo, Y., Mie, M. and Kobatake, E.
    "Design of luciferase-displaying protein nanoparticles for use as highly sensitive immunoassay detection probes"
    Analyst, 141: 6557-6563, (2016)
    Detailed record
  • Mie, M., Naoki, T. and Kobatake, E.
    "Development of a split SNAP-CLIP double labeling system for tracking proteins following dissociation from protein-protein complexes in living cells"
    Anal. Chem., 88(16): 8166–8171, (2016)
    Detailed record
  • Adnan, N., Mie, M., Haque, A., Hossain, S., Mashimo, Y., Akaike, T. and Kobatake, E.
    "Construction of a defined biomimetic matrix for long-term maintenance of mouse induced pluripotent stem cells"
    Bioconjug. Chem., 27(7): 1599-1605, (2016)
    Detailed record
  • Heo, Y., Kim, EH., Kobatake, E., Nah, JW., Ito, Y. and Son, TI.
    "Preparation of phosphonated gelatin-coated titanium containing rhBMP-2 by UV irradiation for improved osteoinduction and function"
    J. Ind. Eng. Chem., 36: 66-73, (2016)
    Detailed record
  • Ibrahim, S., Charinpanitkul, T., Kobatake, E. and Sriyudthsak, M.
    "Nanowires nickel oxide and nano-spherical manganese oxide synthesized via low temperature hydrothermal technique for hydrogen peroxide sensor"
    J. Chem., 2016: Article ID 9138961, (2016)
    Detailed record
  • Siew, S. L., Kaneko, M., Mie, M. and Kobatake, E.
    "Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation"
    J. Mater. Chem. B, 4: 2512-2518, (2016)
    Detailed record

2015

  • Ito, Y., Kobatake, E., Li, J. and Lee, SW.
    "Drug Delivery Using Novel Biological and Synthetic Materials"
    Biomed. Res. Int., 2015: Article ID 682578, (2015)
    Detailed record
  • Suttinont, C., Mashimo, Y., Mie, M., and Kobatake, E.
    "Delivery of bFGF for tissue engineering by tethering to the ECM"
    Biomed. Res. Int., 2015: Article ID 208089, (2015)
    Detailed record
  • Haque, A., Adnan, N., Motazedian, A., Akter, F., Hossain, S., Kutsuzawa, K., Nag, K., Kobatake, E. and Akaike, T.
    "An engineered N-cadherin substrate for differentiation, survival, and selection of pluripotent stem cell-derived neural progenitors"
    PLoS One, 10(8): e0135170, (2015)
    Detailed record
  • Assal, Y., Mizuguchi, Y., Mie, M. and Kobatake, E.
    "Growth factor tethering to protein nanoparticles via coiled-coil formation for targeted drug delivery"
    Bioconjug. Chem., 26(8): 1672-1677, (2015)
    Detailed record
  • Mie, M., Naoki, T. and Kobatake, E.
    "Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system"
    Anal. Biochem., 477: 53-55, (2015)
    Detailed record
  • Li, Z., Kameda, T., Isoshima, T., Kobatake, E., Tanaka, T., Ito, Y. and Kawamoto, M.
    "Solubilization of single-walled carbon nanotubes using a peptide aptamer in water below the critical micelle concentration"
    Langmuir, 31(11): 3482-3488, (2015)
    Detailed record
  • Kamei, K., Mashimo, Y., Koyama, Y., Fockenberg, C., Nakashima, M., Nakajima, M., Li, J. and Chen, Y.
    "3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients"
    Biomed. Microdevices, Published online: 17-Feb, (2015)
    Detailed record
  • Shibata-Seki, T., Tajima, K., Takahashi, H., Seki H., Masai, J., Goto, H., Kobatake, E., Akaike, T. and Itoh, N.
    "AFM characterization of chemically treated corneal cells"
    Anal. Bioanal. Chem., Published online: 30-Jan, (2015)
    Detailed record
  • Funabashi, H., Yanagi, S., Suzuki, S., Mie, M. and Kobatake, E.
    "Assembly of zinc finger motif-fused enzymes on a dsDNA scaffold for catalyzing consecutive reactions with a proximity effect"
    Biotechnol. Lett., 37(1): 109-114, (2015)
    Detailed record

2014

  • Mie, M., Sasaki, S. and Kobatake, E.
    "Construction of a bFGF-tethered multi-functional extracellular matrix protein through coiled-coil structures for neurite outgrowth induction"
    Biomed. Mater., 9(1): 15004, (2014)
    Detailed record
  • Li, Z., Uzawa, T., Zhao, H., Luo, S.C., Yu, H.H., Kobatake, E. and Ito, Y.
    "In vitro selection of peptide aptamers using a ribosome display for a conducting polymer"
    J. Biosci. Bioeng., 117(4): 501-503, (2014)
    Detailed record
  • Akter, F., Mie, M. and Kobatake, E.
    "DNA-based immunoassays for sensitive detection of protein"
    Sensors and Actuators B: Chemical, 202: 1248-1256, (2014)
    Detailed record
  • Usui, K., Kikuchi, T., Kikuchi, K., Mie, M., Kobatake, E. and Mihara, H.
    "Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides"
    Bioorg. Med. Chem. Lett., 24(17): 4129-4131, (2014)
    Detailed record
  • Matsumoto, R., Hara, R., Andou, T., Mie, M. and Kobatake, E.
    "Targeting of EGF-displayed protein nanoparticles with anticancer drugs"
    J. Biomed. Mater. Res. B Appl. Biomater., 102(8): 1792-1798, (2014)
    Detailed record

2013

  • Bae, J., Mie, M. and Kobatake, E.
    "Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein"
    Biotechnol. Lett., 35(12): 2081-2089, (2013)
    Detailed record
  • Usui, K., Kikuchi, T., Mie, M., Kobatake, E. and Mihara, H.
    "Systematic screening of the cellular uptake of designed alpha-helix peptides"
    Bioorg. Med. Chem., 21(9): 2560-2567, (2013)
    Detailed record
  • Assal, Y., Mie, M. and Kobatake, E.
    "The promotion of angiogenesis by growth factors integrated with ECM proteins through coiled-coil structures"
    Biomaterials, 34(13): 3315-3323., (2013)
    Detailed record
  • Mie, M., Kai, T., Le, T., Cass, A.E. and Kobatake, E.
    "Selection of DNA aptamers with affinity for pro-gastrin-releasing peptide (proGRP) a tumor marker for small cell lung cancer"
    Appl. Biochem. Biotechnol., 169(1): 250-255, (2013)
    Detailed record
  • Mie, M., Omuro, M. and Kobatake, E.
    "Hydrogel scaffolds composed of genetically synthesized self-assembling peptides for three-dimentional cell culture"
    Polymer J., 45(5): 504-508, (2013)
    Detailed record
  • Kakiyama, T., Usui, K., Tomizaki, K., Mie, M., Kobatake, E. and Mihara, H.
    "A peptide release system using a photo-cleavable linker in a cell array format for cell-toxicity analysis"
    Polymer J., 45(5): 535-539, (2013)
    Detailed record
  • Li, Z., Uzawa, T., Tanaka, T., Hida, A., Ishibashi, K., Katakura, H., Kobatake, E. and Ito, Y.
    "In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display"
    Biotechnol. Lett., 35(1): 39-45, (2013)
    Detailed record

2012

  • Tada, S., Andou, T., Suzuki, T., Dohmae, N., Kobatake, E. and Ito, Y.
    "Genetic PEGylation"
    PLoS One, 7(11): e49235, (2012)
    Detailed record
  • Bae, J., Mie, M. and Kobatake, E.
    "Targeted gene delivery via PEI complexed with an antibody"
    Appl. Biochem. and Biotechnol., 168(8): 2184-2190, (2012)
    Detailed record
  • Mie, M., Kaneko, M., Henmi, F. and Kobatake, E.
    "Induction of motor neuron differentiation by transduction of Olig2 protein"
    Biochem. Biophys. Res. Commun., 427(3): 531-536, (2012)
    Detailed record
  • Akter, F., Mie, M. and Kobatake, E.
    "Aptamer-based protein detection using a bioluminescent fusion protein"
    Analyst, 137(22): 5297-5301, (2012)
    Detailed record
  • Mie, M., Naoki, T., Uchida, K. and Kobatake, E.
    "Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells"
    Analyst, 137(20): 4760-4765, (2012)
    Detailed record
  • Akter, F., Mie, M., Grimm, S., Nygren, P.A. and Kobatake, E.
    "Detection of Antigens Using a Protein-DNA Chimera Developed by Enzymatic Covalent Bonding with phiX Gene A"
    Anal. Chem. , 84(11): 5040-5046, (2012)
    Detailed record
  • Mashimo, Y., Maeda, H., Mie, M. and Kobatake, E.
    "Construction of Semisynthetic DNA-Protein Conjugates with Phi X174 Gene-A* Protein"
    Bioconjug. Chem., 23(6): 1349-1355, (2012)
    Detailed record
  • Kobatake, E. , Kosaku, C., Hanzawa, S. and Mie, M.
    "Construction of affinity changeable antibody in response to Ca(2+)"
    Biotechnol. Lett. , 34(6): 1019-1023, (2012)
    Detailed record
  • Mie, M., Thuy, N.P.B. and Kobatake, E.
    "Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase" Analyst, 137(5): 1085-1089, (2012)
    Detailed record
  • Nakamura, M., Mie, M., Nakamura, M. and Kobatake, E.
    "Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells"
    Biotechnol. Lett., 34(8): 1571-1577, (2012)
    Detailed record

2011

  • Yamamoto, K., Furuya, K., Nakamura, M., Kobatake, E., Sokabe, M. and Ando, J.
    "Visualization of flow-induced ATP release and triggering of Ca2+ waves at caveolae in vascular endothelial cells"
    J. Cell Sci., 124(Pt 20): 3477-3483, (2011)
    Detailed record
  • Usui, K., Kakiyama, T., Tomizaki, K.Y., Mie, M., Kobatake, E. and Mihara, H.
    "Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity"
    Bioorg. Med. Chem. Lett., 21(21): 6281-6284, (2011)
    Detailed record
  • Montalbetti, N., Leal Denis, M.F., Pignataro, O.P., Kobatake, E., Lazarowski, E.R. and Schwarzbaum, P.J.
    "Homeostasis of extracellular ATP in human erythrocytes"
    J. Biol. Chem., 286(44): 38397-38407, (2011)
    Detailed record
  • Kobatake, E., Takahashi, R. and Mie, M.
    "Construction of a bFGF-tethered extracellular matrix using a coiled-coil helical interaction"
    Bioconjug. Chem., 22(10): 2038-2042, (2011)
    Detailed record
  • Mashimo, Y., Mie, M., Suzuki, S. and Kobatake, E.
    "Detection of small RNA molecules by a combination of branched rolling circle amplification and bioluminescent pyrophosphate assay" Anal. Bioanal. Chem., 401(1): 221-227, (2011)
    Detailed record
  • Akter, F., Mie, M. and Kobatake, E.
    "Immuno-rolling circle amplification using a multi-binding fusion protein"
    Anal. Biochem., 416: 174-179, (2011)
    Detailed record
  • Andou, T., Endoh, T., Mie, M. and Kobatake, E.
    "Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase"
    Analyst, 136(12): 2446-2449, (2011)
    Detailed record
  • Kunii, T., Ogura, S., Mie, M. and Kobatake, E.
    "Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX"
    Analyst, 136: 1310-1312, (2011)
    Detailed record
  • Andou, T., Endoh, T., Mie, M. and Kobatake, E.
    "Development of an RNA detection system using bioluminescence resonance energy transfer"
    Sensors and Actuators B, 152: 277-284, (2011)
    Detailed record
  • Nakamura, M., Mie, M. and Kobatake, E.
    "Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains"
    Analyst, 136: 71-72, (2011)
    Detailed record
  • Kobatake, E., Yamano, R. and Mie, M.
    "Targeted delivery using immunoliposomes with a lipid-modified antibody-binding protein"
    Appl. Biochem. Biotechnol., 163: 296-303, (2011)
    Detailed record

2010

  • Mie, M., Andou, T., Thuy, N.P.B., Endoh, T. and Kobatake, E.
    "Development of Renilla luciferase complementation system for homogeneous assay"
    LUMINESCENCE , 25(2): 169-170, (2010)
    Detailed record
  • Suzuki, M., Takahashi, T., Sato, J., Mie, M., Kobatake, E. and Mihara, H.
    "Designed short peptides that form amyloid-like fibrils in coassembly with amyloid β-petide (Aβ) decrease the toxicity of Aβ to neuronal PC12 cells"
    Chem. Bio. Chem., 11(11): 1525-1530, (2010)
    Detailed record
  • Bae, J., Goto, S., Mie, M. and Kobatake, E.
    "Construction of a thermostable cell adhesion protein for reverse transfection"
    J. Biotechnol., 150: 447-51, (2010)
    Detailed record
  • Mie, M., Sugita, R., Endoh, T. and Kobatake, E.
    "Evaluation of small ligand-protein interactions by using T7 RNA polymerase with DNA-modified ligand"
    Anal. Biochem., 405: 109-13, (2010)
    Detailed record
  • Sugita, R ., Mie, M., Funabashi, H. and Kobatake, E.
    "Evaluation of small ligand-protein interaction by ligation reaction with DNA-modified ligand"
    Biotechnol. Lett., 32: 97-102, (2010)
    <Detailed record

2009

  • Endoh, T., Shintani, R., Mie, M., Kobatake, E., Ohtsuki, T. and Sisido, M.
    "Detection of bioactive small molecules by fluorescent resonance energy transfer in RNA-protein conjugate"
    Bioconjug. Chem., 20(12): 2242-2246, (2009)
    Detailed record
  • Shi, X.W., Yang, X., Gaskell, K.J., Liu, Y., Kobatake, E., Bentley, W.E. and Payne, G.F.
    "Reagentless protein assembly triggered by localized electrical signals"
    Advanced Materials, 21(9): 984-988, (2009)
    Detailed record
  • Wu, H.C., Shi, X.W., Tsao, C.Y., Lewandowski, A.T., Fernandes, R., Hung, C.W., DeShong, P., Kobatake, E., Valdes, J.J., Payne, G.F. and Bentley, W.E.
    "Biofabrication of antibodies and antigens via IgG-binding domain engineered with activatable pentatyrosine pro-tag"
    Biotechnol. Bioeng., 103(2): 231-240, (2009)
    Detailed record
  • Nakamura, M., Yamaguchi, K., Mie, M ., Nakamura, M ., Akita, K. and Kobatake, E.
    "Promotion of angiogenesis by an artificial extracellular matrix protein containing the laminin-1-derived IKVAV sequence"
    Bioconjug. Chem., 20: 1759-1764, (2009)
    Detailed record
  • Nakamura, M., Mie, M., Mihara, H., Nakamura, M. and Kobatake, E.
    "Construction of a multi-functional extracellular matrix protein that increases number of N1E-115 neuroblast cells having neurites"
    J. Biomed. Mater. Res. Part B: Appl. Biomater., 91: 425-432, (2009)
    Detailed record
  • Fujita, Y., Mie, M. and Kobatake, E.
    "Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain"
    Biomaterials, 30: 3450-3457, (2009)
    Detailed record
  • Noda, T., Fujino, T., Mie, M. and Kobatake, E.
    "Transduction of MyoD protein into myoblasts induces myogenic differentiation without addition of protein transduction domain"
    Biochem. Biophys. Res. Commun., 382: 473-477, (2009)
    Detailed record
  • Seidi, A., Mie, M. and Kobatake, E.
    "Recombination system based on Cre alpha complementation and leucine zipper fusions"
    Appl. Biochem. Biotechnol., 158: 334-342, (2009)
    Detailed record
  • Elloumi-Hannachi, I., Nakamura, M ., Mie, M. and Kobatake, E.
    "Construction of multifunctional proteins for tissue engineering: epidermal growth factor with collagen binding and cell adhesive activities"
    J. Biotechnol., 139: 19-25, (2009)
    Detailed record
  • Andou, T., Endoh, T., Mie, M. and Kobatake, E.
    "RNA detection using peptide-inserted Renilla luciferase"
    Anal. Bioanal. Chem., 393: 661-668, (2009)
    Detailed record

2008

  • Mie, M., Manabe, M. and Kobatake, E.
    "Combining electrical stimulation and cisplatin treatment increases caspase activity"
    Electrochemistry, 76(8): 529-531, (2008)
    Detailed record
  • Shi, X.W., Wu, H.C., Liu, Y., Tsao, C.Y., Wang, K., Kobatake, E., Bentley, W.E. and Payne, G.F.
    "Chitosan fibers: versatile platform for nickel-mediated protein assembly"
    Biomacromolecules, 9(5): 1417-1423, (2008)
    Detailed record
  • Mie, M., Shimizu, S., Takahashi, F. and Kobatake, E.
    "Selection of mRNA 5'-untranslated region sequence with high translation efficiency through ribosome display"
    Biochem. Biophys. Res. Commun., 373(1): 48-52, (2008)
    Detailed record
  • Nakamura, M., Mie, M ., Mihara, H ., Nakamura, M. and Kobatake, E.
    "Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells"
    Biomaterials, 29(20): 2237-2248, (2008)
    Detailed record
  • Endoh, T. , Mie, M. and Kobatake, E.
    "Direct detection of RNA transcription by FRET imaging using fluorescent protein probe"
    J. Biotechnol., 133(4): 413-417, (2008)
    Detailed record
  • Mie, M., Mizushima, Y. and Kobatake, E.
    "Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein"
    J. Biomed. Mater. Res. B: Appl. Biomater., 86B(1): 283-290, (2008)
    Detailed record

2007

  • Saknan, B.P., Haruyama, T. and Kobatake, E.
    "Electrophysiological methods for drug assessment"
    Asian Biomedicine, 1(3): 256-272, (2007)
    Detailed record
  • Mitomo, H., Shigematsu, H., Kobatake, E., Furusawa, H. and Okahata, Y.
    "IgG binding kinetics to oligo B protein A domains on lipid layers immobilized on a 27 MHz quartz-crystal microbalance"
    J. Mol. Recognit., 20: 83-89, (2007)
    Detailed record
  • Fujita, Y., Funabashi, H., Mie, M. and Kobatake, E.
    "Design of a thermocontrollable protein complex"
    Bioconjug. Chem., 18: 1619-24, (2007)
    Detailed record
  • Seidi, A., Mie, M. and Kobatake, E.
    "Novel recombination system using Cre recombinase alpha complementation"
    Biotechnol. Lett., 29: 1315-22, (2007)
    Detailed record
  • Endoh, T., Mie, M., Funabashi, H., Sawasaki, T., Endo, Y. and Kobatake, E.
    "Construction of intramolecular luciferase complementation probe for detecting specific RNA"
    Bioconjug. Chem., 18: 956-62, (2007)
    Detailed record
  • Funabashi, H., Ubukata, M., Ebihara, T., Aizawa, M., Mie, M. and Kobatake, E.
    "Assessment of small ligand-protein interactions by electrophoretic mobility shift assay using DNA-modified ligand as a sensing probe"
    Biotechnol. Lett., 29: 785-9, (2007)
    Detailed record

2006

  • Wakabayashi, K., Nakagawa, H., Adachi, T., Kii, I., Kobatake, E., Kudo, A. and Ishikawa, T.
    "Identification of cysteine residues critically involved in homodimer formation and protein expression of human ATP-binding cassette transporter ABCG2: a new approach using the flp recombinase system"
    J. Exp. Ther. Oncol., 5(3): 205-222, (2006)
    Detailed record
  • Mie, M., Mori, K., Funabashi, H. and Kobatake, E.
    "Delivery of antibody-captured proteins into living cells using PTD-fused protein A"
    Biotechnol. Lett., 28: 1209-14, (2006)
    Detailed record
  • Noda, T., Kawamura, R., Funabashi, H., Mie, M. and Kobatake, E.
    "Transduction of NeuroD2 protein induced neural cell differentiation"
    J. Biotechnol., 126(2): 230-236, (2006)
    Detailed record
  • Ihara, H., Mie, M., Funabashi, H., Takahashi, F., Sawasaki, T., Endo, Y. and Kobatake, E.
    "In vitro selection of zinc finger DNA-binding proteins through ribosome display"
    Biochem. Biophys. Res. Commun., 345: 1149-54, (2006)
    Detailed record
  • Funabashi, H., Matsuzawa, R., Nakamura, M., Mie, M. and Kobatake, E.
    "Bioluminescent enumeration of surface antigen-specific cells using the streptavidin-luciferase fusion protein"
    Sensors and Actuators B: Chemical, 120(1): 51-56, (2006)
    Detailed record
  • Nakamura, M., Mie, M., Funabashi, H., Yamamoto, K., Ando, J. and Kobatake, E.
    "Cell-surface-localized ATP detection with immobilized firefly luciferase"
    Anal. Biochem., 352: 61-67, (2006)
    Detailed record
  • Elloumi-Hannachi, I., Kobayashi, R., Funabashi, H., Mie, M. and Kobatake, E.
    "Construction of epidermal growth factor fusion protein with cell adhesive activity"
    Biomaterials, 27: 3451-3458, (2006)
    Detailed record
  • Tanaka, G., Funabashi, H., Mie, M. and Kobatake, E.
    "Fabrication of an antibody microwell array with self-adhering antibody binding protein"
    Anal. Biochem., 350: 298-303, (2006)
    Detailed record
  • Funabashi, H., Tanaka, Y., Imamura, Y., Mie, M.,Manabe, T., Tanaka, H., Takahashi, T., Handa, H., Aizawa, M. and Kobatake, E.
    "Glucose oxidase assisted homogeneous electrochemical receptor binding assay for drug screening"
    Biosens. Bioelectron., 21: 1675-83, (2006)
    Detailed record

2005

  • Takahashi, F., Funabashi, H., Mie, M., Endo, Y., Sawasaki, T., Aizawa, M. and Kobatake, E.
    "Activity-based in vitro selection of T4 DNA ligase"
    Biochem. Biophys. Res. Commun., 336: 987-993, (2005)
    Detailed record
  • Endoh, T., Funabashi, H., Mie, M. and Kobatake, E.
    "Method for detection of specific nucleic acids by recombinant protein with fluorescent resonance energy transfer"
    Anal. Chem., 77: 4308-14, (2005)
    Detailed record
  • Funabashi, H., Ishikawa, M., Mie, M., Takahashi, F., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Electrochemical evaluation of cellular physiological status under stress in Escherichia coli with the rpoS-lacZ reporter gene"
    Biotechnol. Bioeng., 90: 509-515, (2005)
    Detailed record
  • Seino, A., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Translational control by internal ribosome entry site in Saccharomyces cerevisiae"
    Biochim. Biophys. Acta., 1681: 166-174, (2005)
    Detailed record

2004

  • Mei-Kwei, J.S., Kuraoka, I., Horibata, K., Ubukata, M., Kobatake, E., Iwai, S., Handa, H. and Tanaka, K.
    "Blockage of RNA polymerase II at a cyclobutane pyrimidine dimmer and 6-4 photoproduct"
    Biochem. Biophys. Res. Commun., 320: 1133-1138, (2004)
    Detailed record
  • Kobatake, E., Mie, M. and Aizawa, M.
    "Design of functional protein materials self-assembled on solid-phase surface"
    Materials Technology, 19(1): 27-32, (2004)
    Detailed record
  • Kamei, K., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Construction and use of an electrochemical NO sensor in a cell-based assessing system"
    Sensors and Actuators B: Chemical, 99: 106-112, (2004)
    Detailed record
  • Nakamura, M., Mie, M., Funabashi, H. and Kobatake, E.
    "Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form"
    Biotechnol. Lett., 26: 1061-1066, (2004)
    Detailed record
  • Wicaksono, D.H ., Ebihara, T. , Funabashi, H., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "On-chip biosensing of estrogen receptor-alpha at single molecular level"
    Biosens. Bioelectron., 19: 1573-1579, (2004)
    Detailed record
  • Manabe, M., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Combined effect of electrical stimulation and cisplatin in HeLa cell death"
    Biotechnol. Bioeng., 86: 661-666, (2004)
    Detailed record
  • Kamei, K., Haruyama, T., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "The construction of endothelial cellular biosensing system for the control of blood pressure drugs"
    Biosens. Bioelectron., 19: 1121-1124, (2004)
    Detailed record

2003

  • Wada, A., Mie, M., Aizawa, M., Lahoud, P., Cass, A.E. and Kobatake, E.
    "Design and construction of glutamine binding proteins with a self-adhering capability to unmodified hydrophobic surfaces as reagentless fluorescence sensing devices"
    J. Am. Chem. Soc., 125(52): 16228-16234, (2003)
    Detailed record
  • Mie, M., Takahashi, F., Funabashi, H., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Intracellular delivery of antibodies using TAT fusion protein A"
    Biochem. Biophys. Res. Commun., 310(3): 730-734, (2003)
    Detailed record
  • Kobatake, E., Suzuki, S. and Aizawa, M.
    "Assembling of recombinant calmodulin with modulating function on solid surface"
    Sensors and Actuators B: Chemical, 94(2): 165-168, (2003)
    Detailed record
  • Mizuno, A., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "The secretory response through electric stimulation of differentiated PC12 rat pheochromocytoma cells transfected with neuropeptide Y fused with enhanced green fluorescent protein"
    Biotechnol. Lett., 25(7): 547-552, (2003)
    Detailed record
  • Kamei, K. , Haruyama, T., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Development of immune cellular biosensing system for assessing chemicals on iNOS signaling activator"
    Anal. Biochem., 320(1): 75-81, (2003)
    Detailed record
  • Haruyama, T., Bongsebandhu-Phubhakdi, S., Nakamura, I., Mottershead, D., Keinanen, K., Kobatake, E. and Aizawa, M.
    "A biosensing system based on extracellular potential recording of ligand-gated ion channel function overexpressed in insect cells"
    Anal. Chem., 75(4): 918-921, (2003)
    Detailed record
  • Mitomo, H., Kato, R., Ito, A., Kasamatsu, S., Ikegami, Y., Kii, I., Kudo, A., Kobatake, E., Sumino, Y. and Ishikawa, T.
    "A functional study on polymorphism of the ATP-binding cassette transporter ABCG2: critical role of arginine-482 in methotrexate transport"
    Biochem. J., 373(Pt 3): 767-774, (2003)
    Detailed record
  • Mie, M., Endoh, T., Yanagida, Y., Kobatake, E., and Aizawa, M.
    "Induction of neural differentiation by electrically stimulated gene expression of NeuroD2"
    J. Biotechnol., 100(3): 231-238, (2003)
    Detailed record
  • Kamei, K., Haruyama, T., Mie, M., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade"
    Biotechnol. Lett., 25(4): 321-325, (2003)
    Detailed record

2002

  • Seong, G.H. , Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Atomic force microscopy identification of transcription factor NFkappaB bound to streptavidin-pin-holding DNA probe"
    Anal. Biochem., 309: 241-247, (2002)
    Detailed record
  • Funabashi, H., Haruyama, T., Mie, M., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Non-destructive monitoring of rpoS promoter activity as stress marker for evaluating cellular physiological status"
    J. Biotechnol., 95: 85-93, (2002)
    Detailed record
  • Takahashi, F., Ebihara, T., Mie, M., Yanagida, Y., Endo, Y., Kobatake, E. and Aizawa, M.
    "Ribosome display for selection of active dihydrofolate reductase mutants using immobilized methotrexate on agarose beads"
    FEBS Lett., 514: 106-110, (2002)
    Detailed record
  • Haruyama, T., Kobatake, E. and Aizawa, M.
    "Cellular biosensing system for discovery of protein synthesis inhibitors with an electrochemical phosphate modulator to regulate the acid phosphatase gene expression of Saccharomyces cerevisiae"
    Biosens. Bioelectron., 17: 209-215, (2002)
    Detailed record
  • Seong, G.H., Kobatake, E., Miura, K., Nakazawa, A. and Aizawa, M.
    "Direct atomic force microscopy visualization of integration host factor-induced DNA bending structure of the promoter regulatory region on the Pseudomonas TOL plasmid"
    Biochem. Biophys. Res. Commun., 291: 361-366, (2002)
    Detailed record
  • Ebihara, T., Aizawa, M. and Kobatake, E.
    "Direct sensing of ligand-protein interaction by counting of bioaffinity beads with atomic force microscopy"
    Biotechnol. Lett., 24: 1605-1609, (2002)
    Detailed record
  • Koyama, S., Horii, M., Yanagida, Y., Kobatake, E., Miwa, T. and Aizawa, M.
    "Pressure sensitivity of HSP70 promoter in transformed 3T3-L1 cells"
    JAMSTEC J. Deep Sea Res., 20: 53-58, (2002)
    Detailed record
  • Kobatake, E., Seong, G.H., Miura, K., Nakazawa, A. and Aizawa, M.
    "Imaging of IHF-induced DNA bending structure by AFM"
    Single Mol., 3: 165-166, (2002)
    Detailed record
  • Funabashi, H., Mie, M., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Fluorescent monitoring of cellular physiological status depending on the accumulation of ppGpp"
    Biotechnol. Lett., 24: 269-273, (2002)
    Detailed record
  • Ebihara, T., Takayama, H., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Thermostabilization of protein A-luciferase fusion protein by single amino acid mutation"
    Biotechnol. Lett., 24: 147-149, (2002)
    Detailed record

2001

  • Kim, E.J., Yanagida, Y., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Immunosensing system for a-fetoprotein coupled with a disposable amperometric glucose oxidase sensor"
    Sensors and Actuators B: Chemical, 79: 87-91, (2001)
    Detailed record
  • Zhang, C., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Disposable electrochemical capillary-fill device for glucose sensing incorporating a water-soluble enzyme/mediator layer"
    Anal. Chim. Acta., 442: 257-265, (2001)
    Detailed record

2000

  • Sugihara, T., Seong, G.H., Kobatake, E. and Aizawa, M.
    "Genetically synthesized antibody-binding protein self-assembled on hydrophobic matrix"
    Bioconjug. Chem., 11: 789-794, (2000)
    Detailed record
  • Zhang, X.M. , Kobatake, E., Kobayashi, K., Yanagida, Y. and Aizawa, M.
    "Genetically fused protein A-luciferase for immunological blotting analyses"
    Anal. Biochem., 282: 65-69, (2000)
    Detailed record
  • Yanagida, Y., Mizuno, A., Motegi, T., Kobatake, E. and Aizawa, M.
    "Electrically stimulated induction of hsp70 gene expression in mouse astroglia and fibroblast cells"
    J. Biotechnol., 79: 53-61, (2000)
    Detailed record
  • Seong, G.H., Niimi, T., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Single-molecular AFM probing of specific DNA sequencing using RecA-promoted homologous pairing and strand exchange"
    Anal. Chem., 72: 1288-1293, (2000)
    Detailed record
  • Mie, M., Ohgushi, H., Yanagida, Y., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Osteogenesis coordinated in C3H10T1/2 cells by adipogenesis-dependent BMP-2 expression system"
    Tissue Eng., 6: 9-18, (2000)
    Detailed record
  • Kanno, S., Yanagida, Y., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Assembling of engineered IgG-binding protein on gold surface for highly oriented antibody immobilization"
    J. Biotechnol., 76: 207-214, (2000)
    Detailed record
  • Kobatake, E., Yun, K., Yanagida, Y., Haruyama, T., Keinanen, K. and Aizawa, M.
    "Immunoassay systems based on immunoliposomes consisting of genetically engineered single-chain antibody"
    Sensors and Actuators B: Chemical, 65: 42-45, (2000)
    Detailed record
  • Kobatake, E., Onoda, K., Yanagida, Y. and Aizawa, M.
    "Design and gene engineering synthesis of an extremely thermostable protein with biological activity"
    Biomacromolecules, 1: 382-386, (2000)
    Detailed record
  • Ren, X., Kobatake, E. and Aizawa, M.
    "A new type of reusable piezoimmunosensor fabricated by a recombinant IgG-binding protein"
    Analyst, 125: 669-671, (2000)
    Detailed record
  • Afrin, R., Haruyama, T., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Catalytic activity of Teflon particle-immobilized protease in aqueous solution"
    Journal of Molecular Catalysis B: Enzymatic, 9: 259-267, (2000)
    Detailed record
  • Zhang, C., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Evaluation of substituted -1 10-phenanthroline complexes of osmium as mediator for glucose oxidase of Aspergillus Niger"
    Analytica Chimica Acta, 22: 225-232, (2000)
    Detailed record
  • Ebihara, T., Yanagida, Y., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Novel cap-independent translation with the 5'-noncoding region of L-A virus mRNA"
    Biotechnol. Lett., 22: 59-63, (2000)
    Detailed record

Presentation at Conference

2022

  • 第71回高分子学会年次大会@オンライン 2022/05/25-05/27
    ○西田 慶, 三重 正和, 小畠 英理, 田中 賢
    細胞-高分子材料間の相互作用における細胞膜糖鎖の影響
    2022/05/25, 口頭
  • 電気化学会第89回大会@オンライン 2022/03/15-03/17
    〇三重正和、本間彩花、西田慶、小畠英理
    Repを利用したDNA-タンパク質ハイブリッド分子ナノ構造体の構築
    2022/03/15, 口頭
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