KOBATAKE AND MIE LABORATORY

Publications

2018

  • Mie, M., Matsumoto, R., Mashimo, Y., Cass, A.E.G. and Kobatake, E.
    "Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting"
    Mol. Biol. Rep., : 1-9, (2018)
    Detailed record
  • Sugihara, T., Mie, M. and Kobatake, E.
    "Application of elastin-based nanoparticles displaying antibody binding domains for a homogeneous immunoassay"
    Anal. Biochem., 544: 72-79, (2018)
    Detailed record
  • Mashimo, Y., Mie, M. and Kobatake, E.
    "A DNA-scaffold platform enhances a multi-enzymatic cycling reaction"
    Biotechnol. Lett., 40(4): 667–672, (2018)
    Detailed record

2017

  • Mizuguchi, Y., Mashimo, Y., Mie, M. and Kobatake, E.
    "Design of bFGF-tethered self-assembling extracellular matrix proteins via coiled-coil triple-helix formation"
    Biomed. Mater., 12(4): 045021, (2017)
    Detailed record
  • Usui, K., Mie, M., Andou, T., Mihara, H. and Kobatake, E.
    "Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation"
    J. Pept. Sci., 23(7-8): 659-665, (2017)
    Detailed record
  • Kamei, K., Mashimo, Y., Yoshioka, M., Tokunaga, Y., Fockenberg, C., Terada, S., Koyama, Y., Nakajima, M., Shibata‐Seki, T., Liu, L., Akaike, T., Kobatake, E., How, S. E., Uesugi, M. and Chen, Y.
    "Microfluidic‐nanofiber hybrid array for screening of cellular microenvironments"
    Small, 13(18): 1603104, (2017)
    Detailed record

2016

  • Ikeda, Y., Mashimo, Y., Mie, M. and Kobatake, E.
    "Design of luciferase-displaying protein nanoparticles for use as highly sensitive immunoassay detection probes"
    Analyst, 141: 6557-6563, (2016)
    Detailed record
  • Mie, M., Naoki, T. and Kobatake, E.
    "Development of a split SNAP-CLIP double labeling system for tracking proteins following dissociation from protein-protein complexes in living cells"
    Anal. Chem., 88(16): 8166–8171, (2016)
    Detailed record
  • Adnan, N., Mie, M., Haque, A., Hossain, S., Mashimo, Y., Akaike, T. and Kobatake, E.
    "Construction of a defined biomimetic matrix for long-term maintenance of mouse induced pluripotent stem cells"
    Bioconjug. Chem., 27(7): 1599-1605, (2016)
    Detailed record
  • Heo, Y., Kim, EH., Kobatake, E., Nah, JW., Ito, Y. and Son, TI.
    "Preparation of phosphonated gelatin-coated titanium containing rhBMP-2 by UV irradiation for improved osteoinduction and function"
    J. Ind. Eng. Chem., 36: 66-73, (2016)
    Detailed record
  • Ibrahim, S., Charinpanitkul, T., Kobatake, E. and Sriyudthsak, M.
    "Nanowires nickel oxide and nano-spherical manganese oxide synthesized via low temperature hydrothermal technique for hydrogen peroxide sensor"
    J. Chem., 2016: Article ID 9138961, (2016)
    Detailed record
  • Siew, S. L., Kaneko, M., Mie, M. and Kobatake, E.
    "Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation"
    J. Mater. Chem. B, 4: 2512-2518, (2016)
    Detailed record

2015

  • Ito, Y., Kobatake, E., Li, J. and Lee, SW.
    "Drug Delivery Using Novel Biological and Synthetic Materials"
    Biomed. Res. Int., 2015: Article ID 682578, (2015)
    Detailed record
  • Suttinont, C., Mashimo, Y., Mie, M., and Kobatake, E.
    "Delivery of bFGF for tissue engineering by tethering to the ECM"
    Biomed. Res. Int., 2015: Article ID 208089, (2015)
    Detailed record
  • Haque, A., Adnan, N., Motazedian, A., Akter, F., Hossain, S., Kutsuzawa, K., Nag, K., Kobatake, E. and Akaike, T.
    "An engineered N-cadherin substrate for differentiation, survival, and selection of pluripotent stem cell-derived neural progenitors"
    PLoS One, 10(8): e0135170, (2015)
    Detailed record
  • Assal, Y., Mizuguchi, Y., Mie, M. and Kobatake, E.
    "Growth factor tethering to protein nanoparticles via coiled-coil formation for targeted drug delivery"
    Bioconjug. Chem., 26(8): 1672-1677, (2015)
    Detailed record
  • Mie, M., Naoki, T. and Kobatake, E.
    "Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system"
    Anal. Biochem., 477: 53-55, (2015)
    Detailed record
  • Li, Z., Kameda, T., Isoshima, T., Kobatake, E., Tanaka, T., Ito, Y. and Kawamoto, M.
    "Solubilization of single-walled carbon nanotubes using a peptide aptamer in water below the critical micelle concentration"
    Langmuir, 31(11): 3482-3488, (2015)
    Detailed record
  • Kamei, K., Mashimo, Y., Koyama, Y., Fockenberg, C., Nakashima, M., Nakajima, M., Li, J. and Chen, Y.
    "3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients"
    Biomed. Microdevices, Published online: 17-Feb, (2015)
    Detailed record
  • Shibata-Seki, T., Tajima, K., Takahashi, H., Seki H., Masai, J., Goto, H., Kobatake, E., Akaike, T. and Itoh, N.
    "AFM characterization of chemically treated corneal cells"
    Anal. Bioanal. Chem., Published online: 30-Jan, (2015)
    Detailed record
  • Funabashi, H., Yanagi, S., Suzuki, S., Mie, M. and Kobatake, E.
    "Assembly of zinc finger motif-fused enzymes on a dsDNA scaffold for catalyzing consecutive reactions with a proximity effect"
    Biotechnol. Lett., 37(1): 109-114, (2015)
    Detailed record

2014

  • Mie, M., Sasaki, S. and Kobatake, E.
    "Construction of a bFGF-tethered multi-functional extracellular matrix protein through coiled-coil structures for neurite outgrowth induction"
    Biomed. Mater., 9(1): 15004, (2014)
    Detailed record
  • Li, Z., Uzawa, T., Zhao, H., Luo, S.C., Yu, H.H., Kobatake, E. and Ito, Y.
    "In vitro selection of peptide aptamers using a ribosome display for a conducting polymer"
    J. Biosci. Bioeng., 117(4): 501-503, (2014)
    Detailed record
  • Akter, F., Mie, M. and Kobatake, E.
    "DNA-based immunoassays for sensitive detection of protein"
    Sensors and Actuators B: Chemical, 202: 1248-1256, (2014)
    Detailed record
  • Usui, K., Kikuchi, T., Kikuchi, K., Mie, M., Kobatake, E. and Mihara, H.
    "Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides"
    Bioorg. Med. Chem. Lett., 24(17): 4129-4131, (2014)
    Detailed record
  • Matsumoto, R., Hara, R., Andou, T., Mie, M. and Kobatake, E.
    "Targeting of EGF-displayed protein nanoparticles with anticancer drugs"
    J. Biomed. Mater. Res. B Appl. Biomater., 102(8): 1792-1798, (2014)
    Detailed record

2013

  • Bae, J., Mie, M. and Kobatake, E.
    "Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein"
    Biotechnol. Lett., 35(12): 2081-2089, (2013)
    Detailed record
  • Usui, K., Kikuchi, T., Mie, M., Kobatake, E. and Mihara, H.
    "Systematic screening of the cellular uptake of designed alpha-helix peptides"
    Bioorg. Med. Chem., 21(9): 2560-2567, (2013)
    Detailed record
  • Assal, Y., Mie, M. and Kobatake, E.
    "The promotion of angiogenesis by growth factors integrated with ECM proteins through coiled-coil structures"
    Biomaterials, 34(13): 3315-3323., (2013)
    Detailed record
  • Mie, M., Kai, T., Le, T., Cass, A.E. and Kobatake, E.
    "Selection of DNA aptamers with affinity for pro-gastrin-releasing peptide (proGRP) a tumor marker for small cell lung cancer"
    Appl. Biochem. Biotechnol., 169(1): 250-255, (2013)
    Detailed record
  • Mie, M., Omuro, M. and Kobatake, E.
    "Hydrogel scaffolds composed of genetically synthesized self-assembling peptides for three-dimentional cell culture"
    Polymer J., 45(5): 504-508, (2013)
    Detailed record
  • Kakiyama, T., Usui, K., Tomizaki, K., Mie, M., Kobatake, E. and Mihara, H.
    "A peptide release system using a photo-cleavable linker in a cell array format for cell-toxicity analysis"
    Polymer J., 45(5): 535-539, (2013)
    Detailed record
  • Li, Z., Uzawa, T., Tanaka, T., Hida, A., Ishibashi, K., Katakura, H., Kobatake, E. and Ito, Y.
    "In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display"
    Biotechnol. Lett., 35(1): 39-45, (2013)
    Detailed record

2012

  • Tada, S., Andou, T., Suzuki, T., Dohmae, N., Kobatake, E. and Ito, Y.
    "Genetic PEGylation"
    PLoS One, 7(11): e49235, (2012)
    Detailed record
  • Bae, J., Mie, M. and Kobatake, E.
    "Targeted gene delivery via PEI complexed with an antibody"
    Appl. Biochem. and Biotechnol., 168(8): 2184-2190, (2012)
    Detailed record
  • Mie, M., Kaneko, M., Henmi, F. and Kobatake, E.
    "Induction of motor neuron differentiation by transduction of Olig2 protein"
    Biochem. Biophys. Res. Commun., 427(3): 531-536, (2012)
    Detailed record
  • Akter, F., Mie, M. and Kobatake, E.
    "Aptamer-based protein detection using a bioluminescent fusion protein"
    Analyst, 137(22): 5297-5301, (2012)
    Detailed record
  • Mie, M., Naoki, T., Uchida, K. and Kobatake, E.
    "Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells"
    Analyst, 137(20): 4760-4765, (2012)
    Detailed record
  • Akter, F., Mie, M., Grimm, S., Nygren, P.A. and Kobatake, E.
    "Detection of Antigens Using a Protein-DNA Chimera Developed by Enzymatic Covalent Bonding with phiX Gene A"
    Anal. Chem. , 84(11): 5040-5046, (2012)
    Detailed record
  • Mashimo, Y., Maeda, H., Mie, M. and Kobatake, E.
    "Construction of Semisynthetic DNA-Protein Conjugates with Phi X174 Gene-A* Protein"
    Bioconjug. Chem., 23(6): 1349-1355, (2012)
    Detailed record
  • Kobatake, E. , Kosaku, C., Hanzawa, S. and Mie, M.
    "Construction of affinity changeable antibody in response to Ca(2+)"
    Biotechnol. Lett. , 34(6): 1019-1023, (2012)
    Detailed record
  • Mie, M., Thuy, N.P.B. and Kobatake, E.
    "Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase" Analyst, 137(5): 1085-1089, (2012)
    Detailed record
  • Nakamura, M., Mie, M., Nakamura, M. and Kobatake, E.
    "Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells"
    Biotechnol. Lett., 34(8): 1571-1577, (2012)
    Detailed record

2011

  • Yamamoto, K., Furuya, K., Nakamura, M., Kobatake, E., Sokabe, M. and Ando, J.
    "Visualization of flow-induced ATP release and triggering of Ca2+ waves at caveolae in vascular endothelial cells"
    J. Cell Sci., 124(Pt 20): 3477-3483, (2011)
    Detailed record
  • Usui, K., Kakiyama, T., Tomizaki, K.Y., Mie, M., Kobatake, E. and Mihara, H.
    "Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity"
    Bioorg. Med. Chem. Lett., 21(21): 6281-6284, (2011)
    Detailed record
  • Montalbetti, N., Leal Denis, M.F., Pignataro, O.P., Kobatake, E., Lazarowski, E.R. and Schwarzbaum, P.J.
    "Homeostasis of extracellular ATP in human erythrocytes"
    J. Biol. Chem., 286(44): 38397-38407, (2011)
    Detailed record
  • Kobatake, E., Takahashi, R. and Mie, M.
    "Construction of a bFGF-tethered extracellular matrix using a coiled-coil helical interaction"
    Bioconjug. Chem., 22(10): 2038-2042, (2011)
    Detailed record
  • Mashimo, Y., Mie, M., Suzuki, S. and Kobatake, E.
    "Detection of small RNA molecules by a combination of branched rolling circle amplification and bioluminescent pyrophosphate assay" Anal. Bioanal. Chem., 401(1): 221-227, (2011)
    Detailed record
  • Akter, F., Mie, M. and Kobatake, E.
    "Immuno-rolling circle amplification using a multi-binding fusion protein"
    Anal. Biochem., 416: 174-179, (2011)
    Detailed record
  • Andou, T., Endoh, T., Mie, M. and Kobatake, E.
    "Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase"
    Analyst, 136(12): 2446-2449, (2011)
    Detailed record
  • Kunii, T., Ogura, S., Mie, M. and Kobatake, E.
    "Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX"
    Analyst, 136: 1310-1312, (2011)
    Detailed record
  • Andou, T., Endoh, T., Mie, M. and Kobatake, E.
    "Development of an RNA detection system using bioluminescence resonance energy transfer"
    Sensors and Actuators B, 152: 277-284, (2011)
    Detailed record
  • Nakamura, M., Mie, M. and Kobatake, E.
    "Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains"
    Analyst, 136: 71-72, (2011)
    Detailed record
  • Kobatake, E., Yamano, R. and Mie, M.
    "Targeted delivery using immunoliposomes with a lipid-modified antibody-binding protein"
    Appl. Biochem. Biotechnol., 163: 296-303, (2011)
    Detailed record

2010

  • Mie, M., Andou, T., Thuy, N.P.B., Endoh, T. and Kobatake, E.
    "Development of Renilla luciferase complementation system for homogeneous assay"
    LUMINESCENCE , 25(2): 169-170, (2010)
    Detailed record
  • Suzuki, M., Takahashi, T., Sato, J., Mie, M., Kobatake, E. and Mihara, H.
    "Designed short peptides that form amyloid-like fibrils in coassembly with amyloid β-petide (Aβ) decrease the toxicity of Aβ to neuronal PC12 cells"
    Chem. Bio. Chem., 11(11): 1525-1530, (2010)
    Detailed record
  • Bae, J., Goto, S., Mie, M. and Kobatake, E.
    "Construction of a thermostable cell adhesion protein for reverse transfection"
    J. Biotechnol., 150: 447-51, (2010)
    Detailed record
  • Mie, M., Sugita, R., Endoh, T. and Kobatake, E.
    "Evaluation of small ligand-protein interactions by using T7 RNA polymerase with DNA-modified ligand"
    Anal. Biochem., 405: 109-13, (2010)
    Detailed record
  • Sugita, R ., Mie, M., Funabashi, H. and Kobatake, E.
    "Evaluation of small ligand-protein interaction by ligation reaction with DNA-modified ligand"
    Biotechnol. Lett., 32: 97-102, (2010)
    <Detailed record

2009

  • Endoh, T., Shintani, R., Mie, M., Kobatake, E., Ohtsuki, T. and Sisido, M.
    "Detection of bioactive small molecules by fluorescent resonance energy transfer in RNA-protein conjugate"
    Bioconjug. Chem., 20(12): 2242-2246, (2009)
    Detailed record
  • Shi, X.W., Yang, X., Gaskell, K.J., Liu, Y., Kobatake, E., Bentley, W.E. and Payne, G.F.
    "Reagentless protein assembly triggered by localized electrical signals"
    Advanced Materials, 21(9): 984-988, (2009)
    Detailed record
  • Wu, H.C., Shi, X.W., Tsao, C.Y., Lewandowski, A.T., Fernandes, R., Hung, C.W., DeShong, P., Kobatake, E., Valdes, J.J., Payne, G.F. and Bentley, W.E.
    "Biofabrication of antibodies and antigens via IgG-binding domain engineered with activatable pentatyrosine pro-tag"
    Biotechnol. Bioeng., 103(2): 231-240, (2009)
    Detailed record
  • Nakamura, M., Yamaguchi, K., Mie, M ., Nakamura, M ., Akita, K. and Kobatake, E.
    "Promotion of angiogenesis by an artificial extracellular matrix protein containing the laminin-1-derived IKVAV sequence"
    Bioconjug. Chem., 20: 1759-1764, (2009)
    Detailed record
  • Nakamura, M., Mie, M., Mihara, H., Nakamura, M. and Kobatake, E.
    "Construction of a multi-functional extracellular matrix protein that increases number of N1E-115 neuroblast cells having neurites"
    J. Biomed. Mater. Res. Part B: Appl. Biomater., 91: 425-432, (2009)
    Detailed record
  • Fujita, Y., Mie, M. and Kobatake, E.
    "Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain"
    Biomaterials, 30: 3450-3457, (2009)
    Detailed record
  • Noda, T., Fujino, T., Mie, M. and Kobatake, E.
    "Transduction of MyoD protein into myoblasts induces myogenic differentiation without addition of protein transduction domain"
    Biochem. Biophys. Res. Commun., 382: 473-477, (2009)
    Detailed record
  • Seidi, A., Mie, M. and Kobatake, E.
    "Recombination system based on Cre alpha complementation and leucine zipper fusions"
    Appl. Biochem. Biotechnol., 158: 334-342, (2009)
    Detailed record
  • Elloumi-Hannachi, I., Nakamura, M ., Mie, M. and Kobatake, E.
    "Construction of multifunctional proteins for tissue engineering: epidermal growth factor with collagen binding and cell adhesive activities"
    J. Biotechnol., 139: 19-25, (2009)
    Detailed record
  • Andou, T., Endoh, T., Mie, M. and Kobatake, E.
    "RNA detection using peptide-inserted Renilla luciferase"
    Anal. Bioanal. Chem., 393: 661-668, (2009)
    Detailed record

2008

  • Mie, M., Manabe, M. and Kobatake, E.
    "Combining electrical stimulation and cisplatin treatment increases caspase activity"
    Electrochemistry, 76(8): 529-531, (2008)
    Detailed record
  • Shi, X.W., Wu, H.C., Liu, Y., Tsao, C.Y., Wang, K., Kobatake, E., Bentley, W.E. and Payne, G.F.
    "Chitosan fibers: versatile platform for nickel-mediated protein assembly"
    Biomacromolecules, 9(5): 1417-1423, (2008)
    Detailed record
  • Mie, M., Shimizu, S., Takahashi, F. and Kobatake, E.
    "Selection of mRNA 5'-untranslated region sequence with high translation efficiency through ribosome display"
    Biochem. Biophys. Res. Commun., 373(1): 48-52, (2008)
    Detailed record
  • Nakamura, M., Mie, M ., Mihara, H ., Nakamura, M. and Kobatake, E.
    "Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells"
    Biomaterials, 29(20): 2237-2248, (2008)
    Detailed record
  • Endoh, T. , Mie, M. and Kobatake, E.
    "Direct detection of RNA transcription by FRET imaging using fluorescent protein probe"
    J. Biotechnol., 133(4): 413-417, (2008)
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  • Mie, M., Mizushima, Y. and Kobatake, E.
    "Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein"
    J. Biomed. Mater. Res. B: Appl. Biomater., 86B(1): 283-290, (2008)
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2007

  • Saknan, B.P., Haruyama, T. and Kobatake, E.
    "Electrophysiological methods for drug assessment"
    Asian Biomedicine, 1(3): 256-272, (2007)
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  • Mitomo, H., Shigematsu, H., Kobatake, E., Furusawa, H. and Okahata, Y.
    "IgG binding kinetics to oligo B protein A domains on lipid layers immobilized on a 27 MHz quartz-crystal microbalance"
    J. Mol. Recognit., 20: 83-89, (2007)
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  • Fujita, Y., Funabashi, H., Mie, M. and Kobatake, E.
    "Design of a thermocontrollable protein complex"
    Bioconjug. Chem., 18: 1619-24, (2007)
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  • Seidi, A., Mie, M. and Kobatake, E.
    "Novel recombination system using Cre recombinase alpha complementation"
    Biotechnol. Lett., 29: 1315-22, (2007)
    Detailed record
  • Endoh, T., Mie, M., Funabashi, H., Sawasaki, T., Endo, Y. and Kobatake, E.
    "Construction of intramolecular luciferase complementation probe for detecting specific RNA"
    Bioconjug. Chem., 18: 956-62, (2007)
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  • Funabashi, H., Ubukata, M., Ebihara, T., Aizawa, M., Mie, M. and Kobatake, E.
    "Assessment of small ligand-protein interactions by electrophoretic mobility shift assay using DNA-modified ligand as a sensing probe"
    Biotechnol. Lett., 29: 785-9, (2007)
    Detailed record

2006

  • Wakabayashi, K., Nakagawa, H., Adachi, T., Kii, I., Kobatake, E., Kudo, A. and Ishikawa, T.
    "Identification of cysteine residues critically involved in homodimer formation and protein expression of human ATP-binding cassette transporter ABCG2: a new approach using the flp recombinase system"
    J. Exp. Ther. Oncol., 5(3): 205-222, (2006)
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  • Mie, M., Mori, K., Funabashi, H. and Kobatake, E.
    "Delivery of antibody-captured proteins into living cells using PTD-fused protein A"
    Biotechnol. Lett., 28: 1209-14, (2006)
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  • Noda, T., Kawamura, R., Funabashi, H., Mie, M. and Kobatake, E.
    "Transduction of NeuroD2 protein induced neural cell differentiation"
    J. Biotechnol., 126(2): 230-236, (2006)
    Detailed record
  • Ihara, H., Mie, M., Funabashi, H., Takahashi, F., Sawasaki, T., Endo, Y. and Kobatake, E.
    "In vitro selection of zinc finger DNA-binding proteins through ribosome display"
    Biochem. Biophys. Res. Commun., 345: 1149-54, (2006)
    Detailed record
  • Funabashi, H., Matsuzawa, R., Nakamura, M., Mie, M. and Kobatake, E.
    "Bioluminescent enumeration of surface antigen-specific cells using the streptavidin-luciferase fusion protein"
    Sensors and Actuators B: Chemical, 120(1): 51-56, (2006)
    Detailed record
  • Nakamura, M., Mie, M., Funabashi, H., Yamamoto, K., Ando, J. and Kobatake, E.
    "Cell-surface-localized ATP detection with immobilized firefly luciferase"
    Anal. Biochem., 352: 61-67, (2006)
    Detailed record
  • Elloumi-Hannachi, I., Kobayashi, R., Funabashi, H., Mie, M. and Kobatake, E.
    "Construction of epidermal growth factor fusion protein with cell adhesive activity"
    Biomaterials, 27: 3451-3458, (2006)
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  • Tanaka, G., Funabashi, H., Mie, M. and Kobatake, E.
    "Fabrication of an antibody microwell array with self-adhering antibody binding protein"
    Anal. Biochem., 350: 298-303, (2006)
    Detailed record
  • Funabashi, H., Tanaka, Y., Imamura, Y., Mie, M.,Manabe, T., Tanaka, H., Takahashi, T., Handa, H., Aizawa, M. and Kobatake, E.
    "Glucose oxidase assisted homogeneous electrochemical receptor binding assay for drug screening"
    Biosens. Bioelectron., 21: 1675-83, (2006)
    Detailed record

2005

  • Takahashi, F., Funabashi, H., Mie, M., Endo, Y., Sawasaki, T., Aizawa, M. and Kobatake, E.
    "Activity-based in vitro selection of T4 DNA ligase"
    Biochem. Biophys. Res. Commun., 336: 987-993, (2005)
    Detailed record
  • Endoh, T., Funabashi, H., Mie, M. and Kobatake, E.
    "Method for detection of specific nucleic acids by recombinant protein with fluorescent resonance energy transfer"
    Anal. Chem., 77: 4308-14, (2005)
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  • Funabashi, H., Ishikawa, M., Mie, M., Takahashi, F., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Electrochemical evaluation of cellular physiological status under stress in Escherichia coli with the rpoS-lacZ reporter gene"
    Biotechnol. Bioeng., 90: 509-515, (2005)
    Detailed record
  • Seino, A., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Translational control by internal ribosome entry site in Saccharomyces cerevisiae"
    Biochim. Biophys. Acta., 1681: 166-174, (2005)
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2004

  • Mei-Kwei, J.S., Kuraoka, I., Horibata, K., Ubukata, M., Kobatake, E., Iwai, S., Handa, H. and Tanaka, K.
    "Blockage of RNA polymerase II at a cyclobutane pyrimidine dimmer and 6-4 photoproduct"
    Biochem. Biophys. Res. Commun., 320: 1133-1138, (2004)
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  • Kobatake, E., Mie, M. and Aizawa, M.
    "Design of functional protein materials self-assembled on solid-phase surface"
    Materials Technology, 19(1): 27-32, (2004)
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  • Kamei, K., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Construction and use of an electrochemical NO sensor in a cell-based assessing system"
    Sensors and Actuators B: Chemical, 99: 106-112, (2004)
    Detailed record
  • Nakamura, M., Mie, M., Funabashi, H. and Kobatake, E.
    "Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form"
    Biotechnol. Lett., 26: 1061-1066, (2004)
    Detailed record
  • Wicaksono, D.H ., Ebihara, T. , Funabashi, H., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "On-chip biosensing of estrogen receptor-alpha at single molecular level"
    Biosens. Bioelectron., 19: 1573-1579, (2004)
    Detailed record
  • Manabe, M., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Combined effect of electrical stimulation and cisplatin in HeLa cell death"
    Biotechnol. Bioeng., 86: 661-666, (2004)
    Detailed record
  • Kamei, K., Haruyama, T., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "The construction of endothelial cellular biosensing system for the control of blood pressure drugs"
    Biosens. Bioelectron., 19: 1121-1124, (2004)
    Detailed record

2003

  • Wada, A., Mie, M., Aizawa, M., Lahoud, P., Cass, A.E. and Kobatake, E.
    "Design and construction of glutamine binding proteins with a self-adhering capability to unmodified hydrophobic surfaces as reagentless fluorescence sensing devices"
    J. Am. Chem. Soc., 125(52): 16228-16234, (2003)
    Detailed record
  • Mie, M., Takahashi, F., Funabashi, H., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Intracellular delivery of antibodies using TAT fusion protein A"
    Biochem. Biophys. Res. Commun., 310(3): 730-734, (2003)
    Detailed record
  • Kobatake, E., Suzuki, S. and Aizawa, M.
    "Assembling of recombinant calmodulin with modulating function on solid surface"
    Sensors and Actuators B: Chemical, 94(2): 165-168, (2003)
    Detailed record
  • Mizuno, A., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "The secretory response through electric stimulation of differentiated PC12 rat pheochromocytoma cells transfected with neuropeptide Y fused with enhanced green fluorescent protein"
    Biotechnol. Lett., 25(7): 547-552, (2003)
    Detailed record
  • Kamei, K. , Haruyama, T., Mie, M., Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Development of immune cellular biosensing system for assessing chemicals on iNOS signaling activator"
    Anal. Biochem., 320(1): 75-81, (2003)
    Detailed record
  • Haruyama, T., Bongsebandhu-Phubhakdi, S., Nakamura, I., Mottershead, D., Keinanen, K., Kobatake, E. and Aizawa, M.
    "A biosensing system based on extracellular potential recording of ligand-gated ion channel function overexpressed in insect cells"
    Anal. Chem., 75(4): 918-921, (2003)
    Detailed record
  • Mitomo, H., Kato, R., Ito, A., Kasamatsu, S., Ikegami, Y., Kii, I., Kudo, A., Kobatake, E., Sumino, Y. and Ishikawa, T.
    "A functional study on polymorphism of the ATP-binding cassette transporter ABCG2: critical role of arginine-482 in methotrexate transport"
    Biochem. J., 373(Pt 3): 767-774, (2003)
    Detailed record
  • Mie, M., Endoh, T., Yanagida, Y., Kobatake, E., and Aizawa, M.
    "Induction of neural differentiation by electrically stimulated gene expression of NeuroD2"
    J. Biotechnol., 100(3): 231-238, (2003)
    Detailed record
  • Kamei, K., Haruyama, T., Mie, M., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade"
    Biotechnol. Lett., 25(4): 321-325, (2003)
    Detailed record

2002

  • Seong, G.H. , Yanagida, Y., Aizawa, M. and Kobatake, E.
    "Atomic force microscopy identification of transcription factor NFkappaB bound to streptavidin-pin-holding DNA probe"
    Anal. Biochem., 309: 241-247, (2002)
    Detailed record
  • Funabashi, H., Haruyama, T., Mie, M., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Non-destructive monitoring of rpoS promoter activity as stress marker for evaluating cellular physiological status"
    J. Biotechnol., 95: 85-93, (2002)
    Detailed record
  • Takahashi, F., Ebihara, T., Mie, M., Yanagida, Y., Endo, Y., Kobatake, E. and Aizawa, M.
    "Ribosome display for selection of active dihydrofolate reductase mutants using immobilized methotrexate on agarose beads"
    FEBS Lett., 514: 106-110, (2002)
    Detailed record
  • Haruyama, T., Kobatake, E. and Aizawa, M.
    "Cellular biosensing system for discovery of protein synthesis inhibitors with an electrochemical phosphate modulator to regulate the acid phosphatase gene expression of Saccharomyces cerevisiae"
    Biosens. Bioelectron., 17: 209-215, (2002)
    Detailed record
  • Seong, G.H., Kobatake, E., Miura, K., Nakazawa, A. and Aizawa, M.
    "Direct atomic force microscopy visualization of integration host factor-induced DNA bending structure of the promoter regulatory region on the Pseudomonas TOL plasmid"
    Biochem. Biophys. Res. Commun., 291: 361-366, (2002)
    Detailed record
  • Ebihara, T., Aizawa, M. and Kobatake, E.
    "Direct sensing of ligand-protein interaction by counting of bioaffinity beads with atomic force microscopy"
    Biotechnol. Lett., 24: 1605-1609, (2002)
    Detailed record
  • Koyama, S., Horii, M., Yanagida, Y., Kobatake, E., Miwa, T. and Aizawa, M.
    "Pressure sensitivity of HSP70 promoter in transformed 3T3-L1 cells"
    JAMSTEC J. Deep Sea Res., 20: 53-58, (2002)
    Detailed record
  • Kobatake, E., Seong, G.H., Miura, K., Nakazawa, A. and Aizawa, M.
    "Imaging of IHF-induced DNA bending structure by AFM"
    Single Mol., 3: 165-166, (2002)
    Detailed record
  • Funabashi, H., Mie, M., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Fluorescent monitoring of cellular physiological status depending on the accumulation of ppGpp"
    Biotechnol. Lett., 24: 269-273, (2002)
    Detailed record
  • Ebihara, T., Takayama, H., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Thermostabilization of protein A-luciferase fusion protein by single amino acid mutation"
    Biotechnol. Lett., 24: 147-149, (2002)
    Detailed record

2001

  • Kim, E.J., Yanagida, Y., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Immunosensing system for a-fetoprotein coupled with a disposable amperometric glucose oxidase sensor"
    Sensors and Actuators B: Chemical, 79: 87-91, (2001)
    Detailed record
  • Zhang, C., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Disposable electrochemical capillary-fill device for glucose sensing incorporating a water-soluble enzyme/mediator layer"
    Anal. Chim. Acta., 442: 257-265, (2001)
    Detailed record

2000

  • Sugihara, T., Seong, G.H., Kobatake, E. and Aizawa, M.
    "Genetically synthesized antibody-binding protein self-assembled on hydrophobic matrix"
    Bioconjug. Chem., 11: 789-794, (2000)
    Detailed record
  • Zhang, X.M. , Kobatake, E., Kobayashi, K., Yanagida, Y. and Aizawa, M.
    "Genetically fused protein A-luciferase for immunological blotting analyses"
    Anal. Biochem., 282: 65-69, (2000)
    Detailed record
  • Yanagida, Y., Mizuno, A., Motegi, T., Kobatake, E. and Aizawa, M.
    "Electrically stimulated induction of hsp70 gene expression in mouse astroglia and fibroblast cells"
    J. Biotechnol., 79: 53-61, (2000)
    Detailed record
  • Seong, G.H., Niimi, T., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Single-molecular AFM probing of specific DNA sequencing using RecA-promoted homologous pairing and strand exchange"
    Anal. Chem., 72: 1288-1293, (2000)
    Detailed record
  • Mie, M., Ohgushi, H., Yanagida, Y., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Osteogenesis coordinated in C3H10T1/2 cells by adipogenesis-dependent BMP-2 expression system"
    Tissue Eng., 6: 9-18, (2000)
    Detailed record
  • Kanno, S., Yanagida, Y., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Assembling of engineered IgG-binding protein on gold surface for highly oriented antibody immobilization"
    J. Biotechnol., 76: 207-214, (2000)
    Detailed record
  • Kobatake, E., Yun, K., Yanagida, Y., Haruyama, T., Keinanen, K. and Aizawa, M.
    "Immunoassay systems based on immunoliposomes consisting of genetically engineered single-chain antibody"
    Sensors and Actuators B: Chemical, 65: 42-45, (2000)
    Detailed record
  • Kobatake, E., Onoda, K., Yanagida, Y. and Aizawa, M.
    "Design and gene engineering synthesis of an extremely thermostable protein with biological activity"
    Biomacromolecules, 1: 382-386, (2000)
    Detailed record
  • Ren, X., Kobatake, E. and Aizawa, M.
    "A new type of reusable piezoimmunosensor fabricated by a recombinant IgG-binding protein"
    Analyst, 125: 669-671, (2000)
    Detailed record
  • Afrin, R., Haruyama, T., Yanagida, Y., Kobatake, E. and Aizawa, M.
    "Catalytic activity of Teflon particle-immobilized protease in aqueous solution"
    Journal of Molecular Catalysis B: Enzymatic, 9: 259-267, (2000)
    Detailed record
  • Zhang, C., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Evaluation of substituted -1 10-phenanthroline complexes of osmium as mediator for glucose oxidase of Aspergillus Niger"
    Analytica Chimica Acta, 22: 225-232, (2000)
    Detailed record
  • Ebihara, T., Yanagida, Y., Haruyama, T., Kobatake, E. and Aizawa, M.
    "Novel cap-independent translation with the 5'-noncoding region of L-A virus mRNA"
    Biotechnol. Lett., 22: 59-63, (2000)
    Detailed record
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